Vitamin Analysis

Fat- and water-soluble vitamin quantification by HPLC and LC-MS/MS for foods, premixes and fortified products.

Food & Agricultural Testing

About Vitamin Analysis

Vitamin claims on a label are only as good as the assay behind them, and vitamins are notoriously unstable through processing and storage. CVR Labs quantifies fat-soluble vitamins (A, D, E, K) and water-soluble vitamins (C and the B-complex) in foods, premixes, fortified rice kernels, dairy, beverages and supplements using HPLC and LC-MS/MS calibrated against certified reference standards.

We support both label verification — confirming the declared level is actually present — and stability studies that track vitamin loss across shelf life so overages can be set correctly. For fortification programmes (FRK, fortified atta, edible oil with A and D), accredited vitamin assay is the evidence that the public-health add-on is genuinely delivered.

Vitamin Analysis at CVR Labs Chennai
Most Requested

Signature tests under Vitamin Analysis

The checks clients most often ask for with this service — we build the right sub-panel around your product and standard.

Vitamin A & DVitamin E & KVitamin CB-complex (B1, B2, B3, B6, B9, B12)Folic acidPremix & fortificant assay
Test Coverage

What we analyse under Vitamin Analysis

The parameter groups below are drawn from this service's own accredited scope; the full searchable list follows.

Vitamins degrade — claims drift

Vitamin C, A and folic acid decay through processing and storage; only assay at release and over shelf life proves the claim still holds.

Overages must be set on data

Manufacturers add a vitamin overage to survive shelf life — accredited stability data sets that overage correctly instead of by guesswork.

Fortification needs proof of delivery

FRK, fortified oil and atta must deliver the mandated vitamin level; assay is the evidence the programme requires.

Fat-soluble vitamins (A, D, E, K)

Retinol Acetate(Vitamin A)Retinol Palmitate(Vitamin A)Cholecaliferol(Vitamin D3)Ergocalciferol(Vitamin D2)Alpha-Tocopherol(Vitamin E)Phylloquinonel(Vitamin K1)

B-complex & vitamin C

Riboflavin(Vitamin B2)Niacin(Vitamin B3)Pantothenic acid(Vitamin B5)Pyridoxine(Vitamin B6)Biotin(Vitamin B7)Folic acid(Vitamin B9)Cyanocobalamin(Vitamin B12)Ascorbic acid(Vitamin C)

Fortification screens

Carr-Price test for vitamin A
NABL Scope of Accreditation

Vitamin Analysis — parameters & methods

Parameter / Test Method / Standard
Biotin(Vitamin B7) CVR/INS/SOP/LCMS-MS/010
Cyanocobalamin(Vitamin B12) CVR/INS/SOP/LCMS-MS/010
Folic acid(Vitamin B9) CVR/INS/SOP/LCMS-MS/010
Niacin(Vitamin B3) CVR/INS/SOP/LCMS-MS/010
Pantothenic acid(Vitamin B5) CVR/INS/SOP/LCMS-MS/010
Pyridoxine(Vitamin B6) CVR/INS/SOP/LCMS-MS/010
Riboflavin(Vitamin B2) CVR/INS/SOP/LCMS-MS/010
Alpha-Tocopherol(Vitamin E) CVR/INS/SOP/LCMS-MS/012
Cholecaliferol(Vitamin D3) CVR/INS/SOP/LCMS-MS/012
Ergocalciferol(Vitamin D2) CVR/INS/SOP/LCMS-MS/012
Phylloquinonel(Vitamin K1) CVR/INS/SOP/LCMS-MS/012
Retinol Acetate(Vitamin A) CVR/INS/SOP/LCMS-MS/012
Retinol Palmitate(Vitamin A) CVR/INS/SOP/LCMS-MS/012
Carr-Price test for vitamin A FSSAI Manual of Methods of Analysis of foods (Oils and fats), FSSAI 02.040:2021
Carr Price test for vitamin A FSSAI Manual of Methods of Analysis of foods (Oils and fats), FSSAI 02.040:2021
Ascorbic acid(Vitamin C) CVR/INS/SOP/LCMS-MS/011
Need a parameter that isn't listed? Share your product and requirement and our technical team will confirm the applicable method, standard and turnaround, and advise whether it falls within our NABL scope. Contact our technical team →
Methods & Standards

How Vitamin Analysis is tested

Reference methods applied

LC-MS/MS (CVR/INS/SOP/LCMS-MS/010, /011, /012)FSSAI Manual 02.040:2021 (Carr-Price, Vitamin A)Certified reference standards

Where more than one standard applies, the appropriate method is selected for each sample and regulatory requirement. The B-complex, fat-soluble vitamins (A, D, E, K) and vitamin C are quantified by validated in-house CVR LC-MS/MS SOPs against certified reference standards; the qualitative Carr-Price screen for vitamin A in oils follows the FSSAI Manual of Methods — all within the accredited scope.

Accreditation & Recognition

Accreditations covering Vitamin Analysis

See the Accreditations & Scope page for full certificate details.

NABL
ISO 9001
Samples we accept

Samples we accept for Vitamin Analysis

Fortified foodspremixesFRKsupplementsinfant & dairy productsbeverages
How It Works

From enquiry to accredited report

Request a Quote

Share product, parameters and target standard.

Send Sample

Submit in sealed, labelled packaging or arrange pickup.

Login & Prep

Logged in LIMS with unique IDs and prepared.

Accredited Analysis

Validated NABL-scope methods on advanced instruments.

Test Report

Traceable, accredited report with limit comparison.

FAQ

Vitamin Analysis — frequently asked questions

Is Vitamin Analysis NABL-accredited at CVR Labs?
Yes. Vitamin Analysis is performed under CVR Labs' NABL ISO/IEC 17025:2017 accreditation (Cert. NABLT0626TN18226) at our Chennai laboratory, with traceable, audit-ready reports accepted for compliance and submission.
Which vitamins can you quantify?
Fat-soluble vitamins A, D, E and K and water-soluble vitamin C and the full B-complex including folic acid and B12, by HPLC and LC-MS/MS against certified standards.
Can you run vitamin stability studies?
Yes. We track vitamin levels across real-time or accelerated storage so you can set overages and shelf life on real data.
Do you verify fortified foods?
Yes. Fortified rice kernels, fortified atta, edible oil (A and D) and premixes are assayed to confirm the declared and mandated fortification levels.
What samples do you accept for Vitamin Analysis?
We routinely handle Fortified foods, premixes, FRK, supplements, infant & dairy products, beverages. Share your exact sample type and target standard and our technical team will confirm the required quantity, method and turnaround.
What parameters are included in Vitamin Analysis?
The Vitamin Analysis panel covers 16 accredited parameters such as Biotin(Vitamin B7), Cyanocobalamin(Vitamin B12), Folic acid(Vitamin B9), Niacin(Vitamin B3) and Pantothenic acid(Vitamin B5). The full searchable list is on this page — tell us your target and we'll map the right sub-panel for your sample.

Ready to test with an accredited laboratory?

NABL ISO/IEC 17025:2017 · ISO 9001 · ISO 45001 · CPCB · BIS — trusted since 1999.

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