Vitamin Analysis
Fat- and water-soluble vitamin quantification by HPLC and LC-MS/MS for foods, premixes and fortified products.
About Vitamin Analysis
Vitamin claims on a label are only as good as the assay behind them, and vitamins are notoriously unstable through processing and storage. CVR Labs quantifies fat-soluble vitamins (A, D, E, K) and water-soluble vitamins (C and the B-complex) in foods, premixes, fortified rice kernels, dairy, beverages and supplements using HPLC and LC-MS/MS calibrated against certified reference standards.
We support both label verification — confirming the declared level is actually present — and stability studies that track vitamin loss across shelf life so overages can be set correctly. For fortification programmes (FRK, fortified atta, edible oil with A and D), accredited vitamin assay is the evidence that the public-health add-on is genuinely delivered.

Signature tests under Vitamin Analysis
The checks clients most often ask for with this service — we build the right sub-panel around your product and standard.
What we analyse under Vitamin Analysis
The parameter groups below are drawn from this service's own accredited scope; the full searchable list follows.
Vitamins degrade — claims drift
Vitamin C, A and folic acid decay through processing and storage; only assay at release and over shelf life proves the claim still holds.
Overages must be set on data
Manufacturers add a vitamin overage to survive shelf life — accredited stability data sets that overage correctly instead of by guesswork.
Fortification needs proof of delivery
FRK, fortified oil and atta must deliver the mandated vitamin level; assay is the evidence the programme requires.
Fat-soluble vitamins (A, D, E, K)
B-complex & vitamin C
Fortification screens
Vitamin Analysis — parameters & methods
| Parameter / Test | Method / Standard |
|---|---|
| Biotin(Vitamin B7) | CVR/INS/SOP/LCMS-MS/010 |
| Cyanocobalamin(Vitamin B12) | CVR/INS/SOP/LCMS-MS/010 |
| Folic acid(Vitamin B9) | CVR/INS/SOP/LCMS-MS/010 |
| Niacin(Vitamin B3) | CVR/INS/SOP/LCMS-MS/010 |
| Pantothenic acid(Vitamin B5) | CVR/INS/SOP/LCMS-MS/010 |
| Pyridoxine(Vitamin B6) | CVR/INS/SOP/LCMS-MS/010 |
| Riboflavin(Vitamin B2) | CVR/INS/SOP/LCMS-MS/010 |
| Alpha-Tocopherol(Vitamin E) | CVR/INS/SOP/LCMS-MS/012 |
| Cholecaliferol(Vitamin D3) | CVR/INS/SOP/LCMS-MS/012 |
| Ergocalciferol(Vitamin D2) | CVR/INS/SOP/LCMS-MS/012 |
| Phylloquinonel(Vitamin K1) | CVR/INS/SOP/LCMS-MS/012 |
| Retinol Acetate(Vitamin A) | CVR/INS/SOP/LCMS-MS/012 |
| Retinol Palmitate(Vitamin A) | CVR/INS/SOP/LCMS-MS/012 |
| Carr-Price test for vitamin A | FSSAI Manual of Methods of Analysis of foods (Oils and fats), FSSAI 02.040:2021 |
| Carr Price test for vitamin A | FSSAI Manual of Methods of Analysis of foods (Oils and fats), FSSAI 02.040:2021 |
| Ascorbic acid(Vitamin C) | CVR/INS/SOP/LCMS-MS/011 |
How Vitamin Analysis is tested
Reference methods applied
Where more than one standard applies, the appropriate method is selected for each sample and regulatory requirement. The B-complex, fat-soluble vitamins (A, D, E, K) and vitamin C are quantified by validated in-house CVR LC-MS/MS SOPs against certified reference standards; the qualitative Carr-Price screen for vitamin A in oils follows the FSSAI Manual of Methods — all within the accredited scope.
Accreditations covering Vitamin Analysis
See the Accreditations & Scope page for full certificate details.
Samples we accept for Vitamin Analysis
From enquiry to accredited report
Request a Quote
Share product, parameters and target standard.
Send Sample
Submit in sealed, labelled packaging or arrange pickup.
Login & Prep
Logged in LIMS with unique IDs and prepared.
Accredited Analysis
Validated NABL-scope methods on advanced instruments.
Test Report
Traceable, accredited report with limit comparison.
Vitamin Analysis — frequently asked questions
Is Vitamin Analysis NABL-accredited at CVR Labs?
Which vitamins can you quantify?
Can you run vitamin stability studies?
Do you verify fortified foods?
What samples do you accept for Vitamin Analysis?
What parameters are included in Vitamin Analysis?
Ready to test with an accredited laboratory?
NABL ISO/IEC 17025:2017 · ISO 9001 · ISO 45001 · CPCB · BIS — trusted since 1999.
